Journal: Plant biology (Stuttgart, Germany)

Article Title: The transmembrane domain of the rice small protein OsS1Fa1 is responsible for subcellular localization and drought tolerance.

doi: 10.1111/plb.13711

Figure Lengend Snippet: Fig. 2. Analysis of OsS1Fa1 and OsS1Fa2 expression in rice tissues and detection of the His-tagged recombinant proteins in E. coli. (A) A comparison of the deduced amino acid sequences of OsS1Fa1 and OsS1Fa2. (B) Amino acid sequences of conserved motifs in OsS1Fa1 and OsS1Fa2 are indicated. (C) Total RNA was extracted from leaf, culm, and root tissues of 3-week-old rice seedlings, and the expression levels of OsS1Fa1 and OsS1Fa2 were analysed using qRT-PCR. Data represent mean SD (n = 3). (D) Detection of recombinant His6-OsS1Fa1 and His6-OsS1Fa2 proteins with anti-OsS1Fa1 antibody.

Article Snippet: Rice leaf, culm, and root samples were thoroughly ground, and total RNA was extracted from the ground tissue using the FavorPrepTM Plant Total RNA Mini Kit (Favorgen).

Techniques: Expressing, Recombinant, Comparison, Quantitative RT-PCR

Journal: Plant biology (Stuttgart, Germany)

Article Title: The transmembrane domain of the rice small protein OsS1Fa1 is responsible for subcellular localization and drought tolerance.

doi: 10.1111/plb.13711

Figure Lengend Snippet: Fig. 4. Drought tolerance assay of transgenic Arabidopsis overexpressing OsS1Fa1 and mutant proteins. (A) Examination of OsS1Fa1, OsS1Fa1(TMm1), OsS1Fa1(mNLS ), and OsS1Fa1(K63R) expression in OsS1Fa1-, OsS1Fa1(mNLS)-, OsS1Fa1(K63R)-, and OsS1Fa1(TMm1)-overexpressing Arabidopsis. Total RNA was extracted from the leaves of 15-day-old WT and transgenic Arabidopsis, and then OsS1Fa1, OsS1Fa1(TMm1), OsS1Fa1(mNLS ), OsS1Fa1(K63R) transcripts were examined by RT-PCR using OsS1Fa1- and FLAG-specific primers. (B) Seeds of WT, OsS1Fa1-, OsS1Fa1(mNLS)-, OsS1Fa1(K63R)-, and OsS1Fa1(TMm1)- overexpressing Arabidopsis were grown for 15 days in moist soil, and watering was withheld for 7 days for drought stress treatment. Following the drought stress treatment, the plants were rewatered, and photographs taken after an additional 3 and 5 days.

Article Snippet: Rice leaf, culm, and root samples were thoroughly ground, and total RNA was extracted from the ground tissue using the FavorPrepTM Plant Total RNA Mini Kit (Favorgen).

Techniques: Transgenic Assay, Mutagenesis, Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Article Title: Isolation of extracellular vesicles with combined enrichment methods.

doi: 10.1016/j.jchromb.2021.122604

Figure Lengend Snippet: Fig. 4. Overview of EV isolation methods that were Top10 most frequently applied in the year 2019 for cell culture supernatant (A) and plasma/serum (B). Ab breviations: (diff)UC: differential ultracentrifugation, CCS: cell culture supernatant, DG: density gradient (ultra)centrifugation, EQ: Exoquick (precipitation), EXE: exoEASY (membrane affinity), LSC: low-speed centrifugation (<80 000 g), MC: miRCURY (precipitation), PEG: polyethylene glycol (precipitation), SEC: size-exclusion chromatog raphy, TEI: Total exosome isolation kit (precipitation), UF: ultrafiltration.

Article Snippet: A commercial kit is also already available based on membrane affinity; exoEasy Maxi kit (QIAGEN).

Techniques: Isolation, Cell Culture, Clinical Proteomics, Centrifugation, Membrane